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p c juns73  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc p c juns73
    P C Juns73, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 574 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+c+jun+ser73/Phospho-c-Jun+(Ser73)+XP+Rabbit+mAb/pm41818715-53-31-39
    Average 96 stars, based on 574 article reviews
    p c juns73 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    SDS Page:

    Article Title: Beyond endocrine resistance: estrogen receptor (ESR1) activating mutations mediate chemotherapy resistance through the JNK/c-Jun MDR1 pathway in breast cancer
    Article Snippet: Western blot Cells were harvested, lysed, and the total protein was extracted with radioimmunoprecipitation assay (RIPA) buffer (50mM Tris–HCl pH 7.4, 150mM NaCl, 1% NP-40,0.25% Na-deoxycholate, 1mM EDTA, 1mM NaF), together with a protease and phosphatase inhibitor cocktails (Sigma). .. Lysates were resolved on 10% SDS-PAGE and immunoblotted with the indicated antibodies: β-actin (A5441; sigma, St. Louis, MO); p-JNK (AF1205; R&D systems, Minneapolis, United States), p-c-Jun (ser73) (cat# 3270;cell signaling, Massachusetts, United States), T-JNK (cat# 9252;cell signaling, Massachusetts, United States), anti P-glycoprotein (ab170904; Abcam, Cambridge, United Kingdom), TERK (M-5670; Sigma, St. Louis, MO), c-PARP (cat# 5625; cell signaling, Massachusetts, United States) Chromatin immunoprecipitation (ChIP) assay The assay was performed using Magna ChIP A/G Kit (EMD Millipore Corporation). ..

    Article Title: Beyond endocrine resistance: estrogen receptor (ESR1) activating mutations mediate chemotherapy resistance through the JNK/c-Jun MDR1 pathway in breast cancer.
    Article Snippet: Cells were harvested, lysed, and the total protein was extracted with radioimmunoprecipitation assay (RIPA) buffer (50 mM Tris–HCl pH 7.4, 150 mM NaCl, 1% NP-40,0.25% Na-deoxycholate, 1 mM EDTA, 1 mM NaF), together with a protease and phosphatase inhibitor cocktails (Sigma). .. Lysates were resolved on 10% SDS-PAGE and immunoblotted with the indicated antibodies: β-actin (A5441; sigma, St. Louis, MO); p-JNK (AF1205; R&D systems, Minneapolis, United States), p–c-Jun (ser73) (cat# 3270;cell signaling, Massachusetts, United States), T-JNK (cat# 9252;cell signaling, Massachusetts, United States), anti P-glycoprotein (ab170904; Abcam, Cambridge, United Kingdom), T-ERK (M-5670; Sigma, St. Louis, MO), c-PARP (cat# 5625; cell signaling, Massachusetts, United States). .. The assay was performed using Magna ChIP A/G Kit (EMD Millipore Corporation).

    Chromatin Immunoprecipitation:

    Article Title: Beyond endocrine resistance: estrogen receptor (ESR1) activating mutations mediate chemotherapy resistance through the JNK/c-Jun MDR1 pathway in breast cancer
    Article Snippet: Western blot Cells were harvested, lysed, and the total protein was extracted with radioimmunoprecipitation assay (RIPA) buffer (50mM Tris–HCl pH 7.4, 150mM NaCl, 1% NP-40,0.25% Na-deoxycholate, 1mM EDTA, 1mM NaF), together with a protease and phosphatase inhibitor cocktails (Sigma). .. Lysates were resolved on 10% SDS-PAGE and immunoblotted with the indicated antibodies: β-actin (A5441; sigma, St. Louis, MO); p-JNK (AF1205; R&D systems, Minneapolis, United States), p-c-Jun (ser73) (cat# 3270;cell signaling, Massachusetts, United States), T-JNK (cat# 9252;cell signaling, Massachusetts, United States), anti P-glycoprotein (ab170904; Abcam, Cambridge, United Kingdom), TERK (M-5670; Sigma, St. Louis, MO), c-PARP (cat# 5625; cell signaling, Massachusetts, United States) Chromatin immunoprecipitation (ChIP) assay The assay was performed using Magna ChIP A/G Kit (EMD Millipore Corporation). ..



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